منابع مشابه
Temperature-responsive protein pores.
We describe temperature-responsive protein pores containing single elastin-like polypeptide (ELP) loops. The ELP loops were placed within the cavity of the lumen of the alpha-hemolysin (alphaHL) pore, a heptamer of known crystal structure. The cavity is roughly spherical with a molecular surface volume of about 39,500 A3. In an applied potential, the wild-type alphaHL pore remained open for lon...
متن کاملTemperature-responsive molecular recognition chromatography using phenylalanine and tryptophan derived polymer modified silica beads.
Temperature-responsive polymers incorporating molecular-recognition sites were developed as stationary phases for high-performance liquid chromatography (HPLC). The grafted stationary phases consisted of functional copolymers composed of N-isopropylacrylamide (NIPAAm) and N-acryloyl aromatic amino acid methyl esters, i.e., phenylalanine and tryptophan methyl esters (Phe-OMe and Trp-OMe). Three ...
متن کاملHigh Temperature Gas Chromatography
Gas chromatography (GC) is generally believed to be restricted to the analysis of ‘volatiles’ and is less applicable to the analysis of so-called ‘heavy’ compounds. The introduction of persilylated glass and fused silicea columns, of thermostable stationary phases and of non-discriminative injection devices in capillary GC (CGC) have made the deRnitions of ‘volatile’ and ‘heavy’ very Sexible. H...
متن کاملHigh-temperature ultrafast liquid chromatography.
A novel liquid chromatographic system which enables high temperature ultrafast liquid chromatography (HTU-FLC) has been designed through the careful consideration of heat transfer, band broadening, and pressure drop. Studies of the effect of linear velocity on the HETP show that column efficiency at high velocity, especially of well-retained solutes, dramatically improves at higher temperatures...
متن کاملA temperature responsive biopolymer for mercury remediation.
Tunable biopolymers based on elastin-like polypeptides (ELP) were engineered for the selective removal of mercury. ELP undergoes a reversible thermal precipitation within a wide range of temperatures and was exploited to enable easy recovery of the sequestered mercury. A bacterial metalloregulatory protein, MerR, which binds mercury with an unusually high affinity and selectivity, was fused to ...
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ژورنال
عنوان ژورنال: YAKUGAKU ZASSHI
سال: 1997
ISSN: 0031-6903,1347-5231
DOI: 10.1248/yakushi1947.117.10-11_817